Cat No.
NATE-1054
Description
Acid phosphotase is one of the main enzymes of Mycobacterium tuberculosis metabolism. Acid phosphotase is a probable target for antituberculosis drugs.
Abbr
ACP, Recombinant (E. coli)
Alias
ACP
Species
E. coli
Form
In 3.2 M ammonium sulphate containing 1 mM MgCl2 and 0.1 mM ZnSO4.
Enzyme Commission Number
EC 3.1.3.2
Bio-activity
25 U/mg protein at pH 5.0 and 40°C
Molecular Mass
~ 24,667
Isoelectric point
~ 6.5
Unit Definition
One Unit of acid phosphatase is defined as the amount of enzyme required to produce one μmole of p-nitrophenol from 4-nitrophenyl phosphate per minute at 40°C measured at 410 nm.
Optimum pH
5
Optimum temperature
45°C
Stability
> 2 years at 4°C.
Preparation Instructions
For assay, this enzyme should be diluted in assay buffer containing 0.5 mg/mL BSA, 1 mM magnesium and 0.1 mM zinc. Swirl to mix the enzyme suspension immediately prior to use.
Applications
Acid phosphotase is one of the main enzymes of Mycobacterium tuberculosis metabolism. Acid phosphotase is a probable target for antituberculosis drugs.
Synonyms
EC 3.1.3.2; acid phosphomonoesterase; phosphomonoesterase; glycerophosphatase; acid monophosphatase; acid phosphohydrolase; acid phosphomonoester hydrolase; uteroferrin; acid nucleoside diphosphate phosphatase; orthophosphoric-monoester phosphohydrolase (acid optimum)
