Cat No.
NATE-0102
Description
Carboxypeptidase G is a lysosomal, thiol-dependent protease, which progressively cleaves γ-glutamyl pteroyl poly-γ-glutamate yielding pteroyl-α-glutamate (folic acid) and free glutamate. It is considered highly specific for the γ-glutamyl bond, but not for the C-terminal amino acid of the leaving group.1 Molecular mass of this homodimer is approximately 90 kDa. The enzyme is activated by Zn2+ ions.
Abbr
Carboxypeptidase G, Native (Pseudomonas sp.)
Alias
γ-Glutamyl hydrolase
Species
Pseudomonas sp.
Form
lyophilized powder contains sodium acetate salt.
Enzyme Commission Number
EC 3.4.17.11
Bio-activity
> 3 units/mg protein
CAS No.
9074-87-7
Composition
Protein, ~70% biuret
Unit Definition
One unit will hydrolyze 1.0 μmole of L-glutamic acid from (+)amethopterin per min at pH 7.3 at 30°C.
Applications
Carboxypeptidase G from Pseudomonas sp., or γ-Glutamyl hydrolase, has been used in a study to assess the role of the putidaredoxin COOH-terminus in P-450cam (cytochrome m) hydroxylations. Carboxypeptidase G from Pseudomonas sp. has also been used in a study to investigate the effects of nitric oxide on pemetrexed cytotoxicity via NO?cGMP signaling in lung adenocarcinoma cells.
Synonyms
γ-Glutamyl hydrolase; EC 3.4.17.11; 9074-87-7; glutamate carboxypeptidase; carboxypeptidase G; carboxypeptidase G1; carboxypeptidase G2; glutamyl carboxypeptidase; N-pteroyl-L-glutamate hydrolase
