Cat No.
NATE-1022
Description
Cellulose 1,4-beta-cellobiosidase (non-reducing end) is an enzyme with system name 4-beta-D-glucan cellobiohydrolase (non-reducing end). This enzyme catalyses the following chemical reaction:Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing [cellobiose] from the non-reducing ends of the chains.
Abbr
CBH 2, Recombinant (Microbial)
Alias
CBH 2
Species
Microbial
Form
Suspension in 3.2 M Ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.91
Bio-activity
38 U/mg protein (on 1,4-b-D-cellopentaitol) at pH 5.5 and 40°C
~ 96 U/mg protein (on 1,4-b-D-cellopentaitol) at pH 5.5 and 60°C
CAS No.
37329-65-0
Molecular Mass
~ 42,300
pH Stability
5.0-8.0
Isoelectric point
~ 4.7
Specificity
Hydrolysis of 1,4-beta-D-glucosidic linkages in cellulose and cellooligosaccharides (DP < 4), releasing cellobiose from the non-reducing ends of the chains.
Unit Definition
One Unit of cellobiohydrolase activity is defined as the amount of enzyme required to release one μmole of glucose per minute from 1,4-b-D-cellopentaitol (10 mg/mL) in sodium acetate buffer (100 mM), pH 5.5 at the appropriate temperature.
Optimum pH
5.5
Optimum temperature
60°C
Thermal stability
up to 30°C
Stability
> 4 years at 4°C.
Preparation Instructions
For assay, this enzyme should be diluted in sodium acetate buffer (100 mM), pH 5.5 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Applications
For use in research.
Synonyms
cellulose 1,4-beta-cellobiosidase (non-reducing end); 4-beta-D-glucan cellobiohydrolase (non-reducing end); Cellobiohydrolase II; Cellobiosidase; EC 3.2.1.91; CBH 2; C2 cellulase
