Cat No.
NATE-1201
Description
Chitinase is an extracellular enzyme complex that degrades chitin and has a molecular mass of approximately 30 kDa. Chitin is degraded to N-acetyl-D-glucosamine in 2 enzymatic reactions. Firstly, chitobiose units are removed from chitin by chitodextrinase-chitinase. The second reaction involves N-acetyl-glucosaminidase-chitobiase, which cleaves the disaccharide to its monomer subunits (that comprise of N-acetyl-D-glucosamine). The optimum reaction tempeRature is 37°C.
Abbr
Chitinase, Recombinant (Clostridium thermocellum)
Source
Clostridium thermocellum ATCC 27405
Form
Glycerol/buffer solution
Enzyme Commission Number
EC 3.2.1.14
Bio-activity
25 U/mg
Molecular Mass
43927.1 Da
Purification
> 80 % as judged by SDS-PAGE
Unit Definition
One unit is defined as the amount of enzyme required to release 1μmol of p-nitrophenol per minute from p-nitrophenyl-β-Dtriacetylchitotriose in phosphate-citrate (PC) buffer (50 mM K2HPO4, 12 mM citric acid, pH 6.5) at 60°C.
Optimum pH
6.5 (stable from 4.5 – 6.5)
Optimum temperature
60°C (stable up to 65°C)
Synonyms
Chitinase; chitodextrinase; 1,4-β-poly-N-acetylglucosaminidase; poly-β-glucosaminidase; β-1,4-poly-N-acetyl glucosamidinase; poly[1,4-(N-acetyl-β-D-glucosaminide)] glycanohydrolase; (1->4)-2-acetamido-2-deoxy-beta-D-glucan glycanohydrolase; EC 3.2.1.14
