Cat No.
NATE-0124
Description
Chitinase is an extracellular enzyme complex that degrades chitin and has a molecular mass of approximately 30 kDa. Chitin is degraded to N-acetyl-D-glucosamine in 2 enzymatic reactions. Firstly, chitobiose units are removed from chitin by chitodextrinase-chitinase. The second reaction involves N-acetyl-glucosaminidase-chitobiase, which cleaves the disaccharide to its monomer subunits (that comprise of N-acetyl-D-glucosamine). The optimum reaction tempeRature is 37°C.
Abbr
Chitinase, Native (Trichoderma viride)
Alias
Chitinase
Species
Trichoderma viride
Form
lyophilized powder.
Enzyme Commission Number
EC 3.2.1.14
Bio-activity
> 600 units/g solid
Unit Definition
One unit will liberate 1.0 mg of N-acetyl-D-glucosamine from chitin per hour at pH 6.0 at 25°C in a 2 hour assay. One new 1 hour unit = approx. 50 old 48 hour units.
Applications
Chitinase from Trichoderma viride has been used in a study to investigate the differential release of high mannose structural isoforms by fungal and bacterial endo-β-N-acetylglucosaminidases. Chitinase from Trichoderma viride has also been used in a study to investigate a hevein-like protein and a class I chitinase with antifungal activity from leaves of the paper mulberry. The enzyme from Creative Enzymes has been used to digest chitin in purified sponge spicules during the study of sponge skeletons.
Synonyms
Chitinase; chitodextrinase; 1,4-β-poly-N-acetylglucosaminidase; poly-β-glucosaminidase; β-1,4-poly-N-acetyl glucosamidinase; poly[1,4-(N-acetyl-β-D-glucosaminide)] glycanohydrolase; (1->4)-2-acetamido-2-deoxy-beta-D-glucan glycanohydrolase; EC 3.2.1.14
