Cat No.
NATE-0123
Description
Chitinase is an extracellular enzyme complex that degrades chitin and has a molecular mass of approximately 30 kDa. Chitin is degraded to N-acetyl-D-glucosamine in 2 enzymatic reactions. Firstly, chitobiose units are removed from chitin by chitodextrinase-chitinase. The second reaction involves N-acetyl-glucosaminidase-chitobiase, which cleaves the disaccharide to its monomer subunits (that comprise of N-acetyl-D-glucosamine). The optimum reaction tempeRature is 37°C.
Abbr
Chitinase, Native (Streptomyces griseus)
Alias
Chitinase
Species
Streptomyces griseus
Form
lyophilized powder (essentially salt free).
Enzyme Commission Number
EC 3.2.1.14
Bio-activity
> 200 units/g solid
CAS No.
9001-06-3
Unit Definition
One unit will liberate 1.0 mg of N-acetyl-D-glucosamine from chitin per hour at pH 6.0 at 25°C in a 2 hour assay. One new 1 hour unit = approx. 50 old 48 hour units.
Applications
Chitinase from Streptomyces griseus has been used to study the effect of the allosamidin on the regulatory system for chitinase production. It has also been used to study the enrichment of chitinolytic microorganisms. This enrichment was achieved by the isolation and characterization of a chitinase with antifungal activity against phytopathogenic fungi. The enzyme from Creative Enzymes has been used as a standard for chitinase assays using the enzyme derived from Absidia glauca and Petunia hybrida extracts.
Synonyms
Chitinase; chitodextrinase; 1,4-β-poly-N-acetylglucosaminidase; poly-β-glucosaminidase; β-1,4-poly-N-acetyl glucosamidinase; poly[1,4-(N-acetyl-β-D-glucosaminide)] glycanohydrolase; (1->4)-2-acetamido-2-deoxy-beta-D-glucan glycanohydrolase; EC 3.2.1.14
