Cat No.
NATE-0129
Description
Choloylglycine hydrolase (EC 3.5.1.24) is an N-terminal nucleophilic (Ntn) hydrolase that catalyzes the hydrolysis of amide bonds, libeRates the glycine/taurine moiety from the steroid core and eventually yields unconjugated bile acids. Agents that oxidize thiol groups (e.g., p-mercuribenzoate, iodoacetamide, Hg2+, Cu2+, and Cd2+) have been shown to strongly inhibit bile salt hydrolase (BSH) activity in Clostridium perfringens.
Abbr
Choloylglycine Hydrolase, Native (Clostridium perfringens (C. welchii))
Alias
glycocholase
Species
Clostridium perfringens (C. welchii)
Form
lyophilized powder. Partially purified lyophilized powder containing buffer salts and stabilizer
Enzyme Commission Number
EC 3.5.1.24
Bio-activity
> 100 units/mg protein
CAS No.
37289-07-9
Unit Definition
One unit will hydrolyze 1.0 μmole of glycocholic acid to glycine and cholic acid in 5 min at pH 5.6 at 37°C.
Applications
The enzyme from Creative Enzymes has been used in the analysis of bile samples in various studies.
Synonyms
EC 3.5.1.24; glycocholase; bile salt hydrolase; choloyltaurine hydrolase; 3α,7α,12α-trihydroxy-5β-cholan-24-oylglycine amidohydrolase; 37289-07-9
