Cat No.
NATE-1147
Description
Carboxypeptidase B (or peptidyl-L-lysine (-L-arginine) hydrolase) catalyzes the hydrolysis of the basic amino acids, lysine, arginine, and ornithine from the C-terminal position of polypeptides. It has been shown to be a single polypeptide of 34 kDa Da. Trypsin is capable of converting native enzyme to the active enzyme, carboxypeptidase B II in vitro. The optimum pH is found to be 9.0. The enzyme may be used for sequence analysis by successive cleavage of C-terminal basic amino acids. It can also be used as a serum marker for the diagnosis of acute pancreatitis.
Abbr
CPB1, Recombinant (Porcine)
Alias
CPB1
Species
Porcine
Enzyme Commission Number
EC 3.4.17.2
Bio-activity
>180U/mg
Appearance
White powder, lyophilized
Molecular Mass
About 35kDa (SDS-PAGE detection)
Purification
>90% (SDS-PAGE test)
Unit Definition
One unit will catalyze 1.0 μmole of Hip-L-Arg per min at pH 7.65 at 25°C.
Synonyms
carboxypeptidase B; protaminase; CPB1; pancreatic carboxypeptidase B; tissue carboxypeptidase B; peptidyl-L-lysine [L-arginine]hydrolase; EC 3.4.17.2; 9025-24-5
