Cat No.
NATE-1060
Description
In enzymology, a creatinase (EC 3.5.3.3) is an enzyme that catalyzes the chemical reaction:creatine + H2O? sarcosine + urea. Thus, the two substrates of this enzyme are creatine and H2O, whereas its two products are sarcosine and urea. This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amidines. Creatinase accelerates the conversion reaction of creatine and water molecule to sarcosine and urea. It always acts in homodimer state and is induced by choline chloride.
Abbr
Creatinase, Recombinant (Bacillus sp.)
Species
Bacillus sp.
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.5.3.3
Bio-activity
10 U/mg protein at pH 7.5 and 37°C.
Molecular Mass
~ 47,000
pH Stability
5.0 - 9.0
Isoelectric point
~ 5.5
Unit Definition
One Unit of creatinase is defined as the amount of enzyme required to produce one μmole of creatine to sarcosine and urea per minute monitored at 435 nm.
Optimum pH
7.5 - 8.0
Optimum temperature
37°C - 40°C
Thermal stability
up to 40°C
Stability
> 2 years at 4°C.
Preparation Instructions
For assay, this enzyme should be diluted in Potassium phosphate buffer (10 mM), pH 7.5 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Applications
High purity recombinant Creatinase (Bacillus sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. May be used in the measurement of creatine.
Synonyms
Creatine amidohydrolase; Creatinase; EC 3.5.3.3
