Cat No.
NATE-1242
Description
Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit. It is a cyclic amidohydrolase catalysing the reversible conversion of creatinine to creatine. Each monomer contains a binuclear zinc centre near the C termini of the β-strands and the N termini of the main α-helices. These zinc ions indicate the location of the active site.
Abbr
Creatininase, Recombinant (E. coli)
Source
E. coli
Species
E. coli
Enzyme Commission Number
EC 3.5.2.10
Bio-activity
> 500 U/mg lyophilizate
Appearance
White lyophilizate
Molecular Mass
ca. 170 kDa
pH Stability
7.0–11.0
Michaelis Constant
3.4 x 10^-2 M (creatinine)
4.3 x 10^-2 M (creatine)
Structure
6 subunits of 28 kDa (SDS-PAGE)
Isoelectric point
4.8
Unit Definition
One unit (U) is defined as the amount of enzyme which produces 1 μmol of creatine per min at 37°C and pH 6.8.
Optimum pH
6.5–7.0
Optimum temperature
60–65°C
Activators
Mg2+, Mn2+
Thermal stability
below 60°C
Inhibitors
Hg2+
Synonyms
EC 3.5.2.10, creatinine hydrolase; Creatininase; 9025-13-2
