Cat No.
NATE-0163
Description
Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit. It is a cyclic amidohydrolase catalysing the reversible conversion of creatinine to creatine. Each monomer contains a binuclear zinc centre near the C termini of the β-strands and the N termini of the main α-helices. These zinc ions indicate the location of the active site.
Abbr
Creatininase, Native (microorganisms)
Alias
Creatininase
Species
microorganisms
Form
Lyophilized powder containing sucrose and BSA as stabilizers
Enzyme Commission Number
EC 3.5.2.10
Bio-activity
100-300 units/mg protein
CAS No.
9025-13-2
Molecular Mass
mol wt ~175 kDa
pH Stability
pH 7.5 ? 9.0 (5°C, 16hr)
Michaelis Constant
3.2 x 10￣2M (Creatinine), 5.7 x 10￣2M (Creatine) Structure: 6 subunits per mol of enzyme (One mol of zinc is bound to each subunit)
Isoelectric point
4.7
Unit Definition
One unit will hydrolyze 1.0 mmole of creatinine to creatine per min at pH 8.0 and 25°C
Optimum pH
6.5 ? 7.5
Optimum temperature
70°C
Thermal stability
Below 70°C (pH 7.5, 30 min)
Inhibitors
Ag+, Hg++, N-bromosuccinimide, EDTA
Applications
This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis.
Synonyms
EC 3.5.2.10, creatinine hydrolase; Creatininase; 9025-13-2
