Cat No.
NATE-0164
Description
In enzymology, a creatinine deaminase (EC 3.5.4.21) is an enzyme that catalyzes the chemical reaction: creatinine + H2O ? N-methylhydantoin + NH3. Thus, the two substrates of this enzyme are creatinine and H2O, whereas its two products are N-methylhydantoin and NH3. This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in cyclic amidines. The systematic name of this enzyme class is creatinine iminohydrolase.
Abbr
Creatinine Deiminase, Native
Alias
creatinine desiminase
Species
Microbial
Form
Lyophilized powder containing mannitol as stabilizer
Enzyme Commission Number
EC 3.5.4.21
Bio-activity
> 25 units/mg protein
CAS No.
37289-15-9
Molecular Mass
mol wt ~260 kDa
pH Stability
pH 7.0 – 11.0 (30°C, 20hr)
Michaelis Constant
3.5 x 10￣3M (Creatinine)
Isoelectric point
4.4
Unit Definition
One unit will hydrolyze 1.0 μmole of creatinine to N-methylhydantoin and NH3 per min at pH 7.5 at 37°C in a coupled system with L-glutamic dehydrogenase.
Optimum pH
8.5 – 9.5
Optimum temperature
65 – 75°C
Thermal stability
Below 65°C (pH 7.5, 1hr)
Inhibitors
Ag+,Hg++, o-phenanthroline,monoiodoacetate
Applications
Creatinine deiminase has been used in a study to assess the application of a creatinine-sensitive biosensor for hemodialysis control. Creatinine deiminase has also been used in a study to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples.
Synonyms
EC 3.5.4.21, creatinine hydrolase; creatinine desiminase; creatinine deaminase; 37289-15-9
