Cat No.
NATE-0193
Description
Dispase is a protease which cleaves fibronectin, collagen IV, and to a lesser extent collagen I. It is found in some bacteria and can be isolated from culture filtrates of Bacillus polymyxa. It can be extracted, purified, and used in research. It can be particularly useful to separate embryonic epithelia and mesenchyme. Dispase II is specific for the cleavage of Leucine-Phenylalanine bonds. Dispase is often used to digest adhering primary cells in culture, since this treatment turned out to be milder than trypsin digestion.
Abbr
Dispase, Native (Bacillus polymyxa)
Alias
Dispase
Species
Bacillus polymyxa
Form
powder
Bio-activity
~0.4 unit/mg solid
CAS No.
42613-33-2
Unit Definition
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37°C (color by Folin-Ciocalteu reagent).
Applications
Dispase is a protease which cleaves fibronectin, collagen IV, and to a lesser extent collagen I. It is found in some bacteria and can be isolated from culture filtrates of Bacillus polymyxa. It can be extracted, purified, and used in research. It can be particularly useful to separate embryonic epithelia and mesenchyme. Dispase II is specific for the cleavage of Leucine-Phenylalanine bonds. Dispase is often used to digest adhering primary cells in culture, since this treatment turned out to be milder than trypsin digestion (Sinclair et al., 2013). A recent article also finds that dispase can digest serine-phenylalanine.
Synonyms
Dispase; 42613-33-2; Proteinase; Bacillus polymyxa neutral
