Cat No.
NATE-0199
Description
Deoxyribonuclease I (usually called DNase I), is an endonuclease coded by the human gene DNASE1. DNase I is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and chromatin. In addition to its role as a waste-management endonuclease, it has been suggested to be one of the deoxyribonucleases responsible for DNA fragmentation during apoptosis.
Abbr
DNASE1, Native (Bovine)
Alias
DNASE1; DNase I
Source
Bovine pancreas
Species
Bovine
Form
buffered aqueous glycerol solution.
Enzyme Commission Number
EC 3.1.21.1
CAS No.
9003-98-9
Molecular Mass
mol wt 29.1 kDa
Unit Definition
One unit will cause a ΔA260 of 0.001 per min per mL reaction mixture using calf thymus DNA as substrate.
Applications
Used in molecular biology applications for removing DNA during RNA purification, for preparing DNA for nick translation, and for DNA-protein interaction analysis by footprinting methods.
Gene Name
DNASE1 deoxyribonuclease I [Bos taurus]
Synonyms
DNASE1; deoxyribonuclease I; deoxyribonuclease-1; DNase I; 9003-98-9; EC 3.1.21.1; pancreatic DNase; DNase; thymonuclease, dornase; dornava; dornavac; pancreatic deoxyribonuclease; pancreatic dornase; deoxyribonuclease (pancreatic); pancreatic DNase; DNAase; deoxyribonucleic phosphatase; alkaline deoxyribonuclease; alkaline DNase; endodeoxyribonuclease I; DNA depolymerase; Escherichia coli endonuclease I; deoxyribonuclease A; DNA endonuclease; DNA nuclease
GeneID
282217
mRNA Refseq
NM_174534
Protein Refseq
NP_776959
UniProt ID
P00639
Chromosome Location
chromosome: 25
Function
actin binding; deoxyribonuclease I activity; endodeoxyribonuclease activity
