Cat No.
NATE-0202
Description
Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. In vitro, its optimum pH range is 4.5-5.0. It also acts upon p-nitrophenyl-phosphodiesters at pH 5.6-5.9. The molecular weight is approximately 38 kDa Da.
Abbr
DNASE2, Native (Porcine)
Alias
DNase II alpha; DNASE2
Source
Porcine spleen
Species
Porcine
Form
lyophilized powder. Contains sodium chloride
Enzyme Commission Number
EC 3.1.22.1
Bio-activity
2,000-6,000 Kunitz units/mg protein (biuret)
CAS No.
9025-64-3
Unit Definition
One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25°C; [Mg2+] = 0.83 mM
Applications
DNase II from Creative Enzymes has been used to treat transformed cells during the purification of β-lactamase. It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA). Deoxyribonuclease II from porcine spleen has been used in an immunohistological study of the immune system cells in paraffin-embedded tissues. Deoxyribonuclease II from porcine spleen has also been used in a study to investigate its reassociation with the lysosomal membrane.
Gene Name
DNASE2 deoxyribonuclease II, lysosomal [Sus scrofa]
Synonyms
DNASE2; deoxyribonuclease II; EC 3.1.22.1; 9025-64-3; DNase II; pancreatic DNase II; deoxyribonucleate 3'-nucleotidohydrolase; DNase II; pancreatic DNase II; acid deoxyribonuclease; acid Dnase
GeneID
397398
mRNA Refseq
NM_214196
Protein Refseq
NP_999361
UniProt ID
O62855
Chromosome Location
chromosome: 2
Pathway
Lysosome, organism-specific biosystem; Lysosome, conserved biosystem
Function
deoxyribonuclease II activity
