Cat No.
NATE-0213
Description
Neutrophil elastase is a serine proteinase in the same family as chymotrypsin and has broad substrate specificity. Secreted by neutrophils and macrophages during inflammation, it destroys bacteria and host tissue. It also localizes to Neutrophil extracellular traps (NETs), via its high affinity for DNA, an unusual property for serine proteases.
Abbr
ELA2, Native (Human)
Alias
ELANE; ELA2
Source
Human leuk ocytes
Species
Human
Form
Lyophilized from 0.05 M sodium acetate (pH 5.5) and 0.6 M NaCl
Enzyme Commission Number
EC 3.4.21.37
Bio-activity
> 50 units/mg protein (Bradford)
CAS No.
9004-06-2
Molecular Mass
29 kDa
Isoelectric point
8.77-9.55
Unit Definition
One unit will release one nanomole of p-nitrophenol per sec from Bec-L-alanine p-nitrophenyl ester at pH 6.5 at 37°C.
Applications
Elastase from Creative Enzymes has been used to digest fibronectin. The results were compared with fibronectin digestion by crude human leuk ocyte homogenateto examine the presence of fibronectin peptides in saliva of patients with Sj?gren′s syndrome. It has also been used as a reference to determine the elastase activity in cell lysates. This study examined the effect of all-trans retinoic acid on pr ocoagulant and fibrinolytic activities of cultured blast cells. These blast cells were from patients with acute promyel ocytic leukemia. Elastase from human leuk ocytes has been used in a study that determined that fragments of Nle3-angiotensin (1-7) accelerate healing in dermal models.
Gene Name
ELANE elastase, neutrophil expressed [Homo sapiens]
Synonyms
ELANE; elastase; EC 3.4.21.37; leukocyte elastase; ELA2; elastase 2; neutrophil elaszym; serine elastase; lysosomal elastase; neutrophil elastase; polymorphonuclear leukocyte elastase; elaszym; granulocyte elastase
GeneID
1991
mRNA Refseq
NM_001972
Protein Refseq
NP_001963
MIM
130130
UniProt ID
P08246
Chromosome Location
19p13.3
Pathway
Activation of Matrix Metalloproteinases, organism-specific biosystem; C-MYB transcription factor network, organism-specific biosystem; Degradation of the extracellular matrix, organism-specific biosystem; Extracellular matrix organization, organism-specific biosystem; Systemic lupus erythematosus, organism-specific biosystem; Systemic lupus erythematosus, conserved biosystem; Transcriptional misregulation in cancer, organism-specific biosystem
Function
bacterial cell surface binding; cytokine binding; endopeptidase activity; heparin binding; peptidase activity; protease binding; protein binding; serine-type endopeptidase activity
