Cat No.
NATE-1184
Description
Mannan endo-1,4-beta-mannosidase is an enzyme with system name 4-beta-D-mannan mannanohydrolase. This enzyme catalyses the following chemical reaction: Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Abbr
β-Mannanase, Recombinant (Clostridium thermocellum)
Source
Clostridium thermocellum YS
Form
Supplied in 35 mM HEPES buffer, pH 7.5, containing 750 mM NaCl, 200 mM imidazole, 3.5 mM CaCl2, 0.02 % (w/v) sodium azide and 25% (v/v) glycerol.
Enzyme Commission Number
EC 3.2.1.78
Bio-activity
1500 U/mg
Concentration
1500 U/ml
Molecular Mass
42700 Da
Purification
> 95 % as judged by SDS-PAGE
Unit Definition
One unit is defined as the amount of enzyme required to release 1μmol of mannose-reducing-sugar equivalents per minute from galactomannan in phosphate-citrate (PC) buffer (50 mM K2HPO4, 12 mM citric acid, pH 6.5) at 60 °C.
Optimum pH
6.5 (stable from 6 - 8)
Optimum temperature
65°C (stable up to 70°C)
Synonyms
endo-1,4-β-mannanase; endo-β-1,4-mannase; β-mannanase B; β-1, 4-mannan 4-mannanohydrolase; endo-β-mannanase; β-D-mannanase; mannan endo-1,4-β-mannosidase; 1,4-β-D-mannan mannanohydrolase; EC 3.2.1.78
