Cat No.
NATE-1113
Description
Mannan endo-1,4-beta-mannosidase is an enzyme with system name 4-beta-D-mannan mannanohydrolase. This enzyme catalyses the following chemical reaction: Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Abbr
endo-1,4 β-Mannanase, Recombinant (Thermotoga maritima)
Species
Thermotoga maritima
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.78
Bio-activity
14 U/mg protein at pH 7.0 and 80°C.
Molecular Mass
~ 75370
pH Stability
6.0-8.0
Isoelectric point
~ 5.1
Unit Definition
One Unit of β-mannanase activity is defined as the amount of enzyme required to release one μmole of mannose-reducing-sugar equivalents per minute from low viscosity carob galactomannan (6.25 mg/mL) in 50 mM MOPS buffer (pH 7.0) at 80°C.
Optimum pH
7
Optimum temperature
85°C
Thermal stability
unstable above 80°C
Stability
> 2 years at 4°C
Preparation Instructions
Swirl to mix the enzyme immediately prior to use.
Applications
High purity endo-1,4 beta-Mannanase (T. maritima) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
endo-1,4-β-mannanase; endo-β-1,4-mannase; β-mannanase B; β-1, 4-mannan 4-mannanohydrolase; endo-β-mannanase; β-D-mannanase; mannan endo-1,4-β-mannosidase; 1,4-β-D-mannan mannanohydrolase; EC 3.2.1.78
