Cat No.
NATE-1112
Description
Mannan endo-1,4-beta-mannosidase is an enzyme with system name 4-beta-D-mannan mannanohydrolase. This enzyme catalyses the following chemical reaction: Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Abbr
endo-1,4 β-Mannanase, Recombinant (Cellvibrio japonicus)
Species
Cellvibrio japonicus
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.78
Bio-activity
400 U/mg protein (on carob galactomannan) at pH 7.0 and 40°C; ~ 635 U/mg protein (on carob galactomannan) at pH 7.0 and 50°C.
Molecular Mass
~ 45,200
pH Stability
5.0 - 9.0
Isoelectric point
~ 5.7
Unit Definition
One Unit of mannanase activity is defined as the amount of enzyme required to release one μmole of mannose reducing-sugar equivalents per minute from carob galactomannan (5 mg/mL) in sodium phosphate buffer (100 mM) pH 7.0.
Optimum pH
7
Optimum temperature
50°C
Thermal stability
up to 50°C
Preparation Instructions
For assay, this enzyme should be diluted in sodium phosphate buffer (100 mM), pH 7.0 containing 0.5 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Applications
High purity recombinant endo-1,4 beta-Mannanase (Cellvibrio japonicus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
endo-1,4-β-mannanase; endo-β-1,4-mannase; β-mannanase B; β-1, 4-mannan 4-mannanohydrolase; endo-β-mannanase; β-D-mannanase; mannan endo-1,4-β-mannosidase; 1,4-β-D-mannan mannanohydrolase; EC 3.2.1.78
