Cat No.
NATE-1110
Description
Mannan endo-1,4-beta-mannosidase is an enzyme with system name 4-beta-D-mannan mannanohydrolase. This enzyme catalyses the following chemical reaction: Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.
Abbr
endo-1,4 β-Mannanase, Native (Bacillus sp.)
Species
Bacillus sp.
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.78
Bio-activity
> 50 U/mg
Molecular Mass
30500
pH Stability
6.0-10.0
Isoelectric point
4.6
Unit Definition
One Unit of β-mannanase activity is the amount of enzyme which releases one micromole of mannose-reducing-sugar equivalents per minute from low viscosity carob galactomannan (10 mg/mL) 0.1 M glycine buffer (pH 8.8) at 40°C.
Optimum pH
8.8
Optimum temperature
65°C
Thermal stability
< 60°C
Stability
> 4 years at 4°C
Applications
High purity endo-1,4 beta-Mannanase (Bacillus sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
endo-1,4-β-mannanase; endo-β-1,4-mannase; β-mannanase B; β-1, 4-mannan 4-mannanohydrolase; endo-β-mannanase; β-D-mannanase; mannan endo-1,4-β-mannosidase; 1,4-β-D-mannan mannanohydrolase; EC 3.2.1.78