Cat No.
NATE-1069
Description
Arabinogalactan endo-beta-1,4-galactanase is an enzyme with system name arabinogalactan 4-beta-D-galactanohydrolase. The enzyme specifically hydrolyses (1->4)-beta-D-galactosidic linkages in type I arabinogalactans. This enzyme is isolated from the bacterium Bacillus subtilis.
Abbr
endo-1,4-β-Galactanase, Recombinant (C. thermocellum)
Species
Clostridium thermocellum
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.89
Bio-activity
15 U/mg protein (on potato galactan) at pH 4.5 and 40°C; ~33 U/mg protein (on potato galactan) at pH 4.5 and 60°C.
Molecular Mass
~ 44,600
pH Stability
4.0 - 9.0
Isoelectric point
~ 5.9
Unit Definition
One Unit of galactanase activity is defined as the amount of enzyme required to release one μmole of galactose reducing-sugar equivalents per minute from potato galactan (10 mg/mL) in sodium acetate buffer (100 mM) pH 4.5.
Optimum pH
4.5
Optimum temperature
60°C
Thermal stability
up to 50°C
Stability
> 2 years at 4°C.
Preparation Instructions
For assay, this enzyme should be diluted in sodium acetate buffer (100 mM), pH 4.5 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Applications
High purity recombinant enzyme endo-1,4-beta-Galactanase (Clostridium thermocellum) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
EC 3.2.1.89; endo-1,4-beta-galactanase; galactanase; arabinogalactanase; ganB (gene); Arabinogalactan endo-beta-1,4-galactanase; arabinogalactan 4-beta-D-galactanohydrolase; endo-1,4-β-Galactanase
