Cat No.
NATE-0834
Description
Arabinan endo-1,5-alpha-L-arabinanase is an enzyme with system name 5-alpha-L-arabinan 5-alpha-L-arabinanohydrolase. This enzyme catalyses the following chemical reaction: Endohydrolysis of (1->5)-alpha-arabinofuranosidic linkages in (1->5)-arabinans. This enzyme is most active on linear 1,5-alpha-L-arabinan.
Abbr
endo-1,5-α-Arabinanase, Recombinant (Aspergillus niger)
Species
Aspergillus niger
Form
In 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.2.1.99
Bio-activity
~ 10 U/mg (40°C, pH 4.0 on CM-linear 1,5-α-L-arabinan).
CAS No.
75432-96-1
Molecular Mass
39750 Da
Isoelectric point
~ 3.0
Specificity
Endohydrolysis of (1,5)-α-arabinofuranose linkages in (1,5)-α-arabinans. Acts more slowly on the (1,5)-α-linked arabinan backbone in branched arabinans than on linear arabinans.
Unit Definition
One Unit of arabinanase activity is defined as the amount of enzyme required to release one μmole of arabinose reducing-sugar equivalents per minute from CM-linear 1,5-α-L-arabinan (6?mg/ml) in sodium acetate buffer (200 mM), pH 4.0 at 40°C.
Stability
> 2 years at 4°C. It is stable to repeated freeze/thaw cycles and to lyophilisation. At room temperature there is no loss of activity in 20 h in the pH range 3.0-7.0. the enzyme is stable at 50°C for several hours.
Applications
High purity endo-1,5-α-arabinanase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. Applications established in food and beverage industries, particularly in the reduction of haze in fruit juices and the processing of sugar beet.
Synonyms
EC 3.2.1.99; endo-exo-Arabinanase; endo-1,5-alpha-L-arabinanase; endo-alpha-1,5-arabanase; endo-arabanase; 1,5-alpha-L-arabinan 1,5-alpha-L arabinanohydrolase
