Cat No.
NATE-0835
Description
Arabinan endo-1,5-alpha-L-arabinanase is an enzyme with system name 5-alpha-L-arabinan 5-alpha-L-arabinanohydrolase. This enzyme catalyses the following chemical reaction: Endohydrolysis of (1->5)-alpha-arabinofuranosidic linkages in (1->5)-arabinans. This enzyme is most active on linear 1,5-alpha-L-arabinan.
Abbr
endo-exo-Arabinanase, Recombinant (Cellvibrio japonicus)
Species
Cellvibrio japonicus
Form
In 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.2.1.99
Bio-activity
54 U/mg protein (on debranched arabinan) at pH 7.0 and 40°C.
Molecular Mass
~ 37 kDa
pH Stability
4.0 - 9.0 (> 75% control activity after 24 hours at 4°C)
Isoelectric point
~ 6.6
Unit Definition
One Unit of arabinananase activity is defined as the amount of enzyme required to release one μmole of arabinose reducing-sugar equivalents per minute from debranched arabinan (5 mg/ml) in potassium phosphate buffer (100 mM) pH 7.0.
Optimum pH
7
Optimum temperature
40°C
Preparation Instructions
For assay, this enzyme should be diluted in potassium phosphate buffer (100 mM), pH 7.0 containing 1.0 mg/ml BSA. Swirl to mix the enzyme immediately prior to use.
Applications
High purity recombinant endo-exo-Arabinanase (Cellvibrio japonicus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
EC 3.2.1.99; endo-exo-Arabinanase; endo-1,5-alpha-L-arabinanase; endo-alpha-1,5-arabanase; endo-arabanase; 1,5-alpha-L-arabinan 1,5-alpha-L arabinanohydrolase
