Cat No.
NATE-1412
Description
Endo-β-Galactosidase is an enzyme that hydrolyzes internal β-galactosidic linkages of oligosaccharides in poly-N-acetyl-lactosamine structures. This enzyme resembles the Escherichia freundii enzyme due to its specificity towards bovine corneal keratan sulphate, milk oligosaccharides and the glycolipids lacto-N-neotetraosylceramide and lacto-N-tetraosylceramide.
Abbr
N-Acetylglucosamine endo-β-galactosidase 16C, Recombinant (Clostridium perfringens)
Source
E. coli
Species
Clostridium perfringens
Form
35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol
Enzyme Commission Number
EC 3.2.1.-
Concentration
1 mg/mL
Molecular Mass
33.7 kDa
Purification
>90% by SDS-PAGE
Specificity
GlcNAc-α-1,4-Gal-β-1,3-GalNAc-α1-Ser/Thr
Optimum pH
6
Optimum temperature
37 °C
Synonyms
β-Galactosidase bacterial; Keratanase; Endo-β-galactosidase; keratan sulfate endogalactosidase; keratan-sulfate 1,4-β-D-galactanohydrolase; EC 3.2.1.103
