Cat No.
NATE-1203
Description
An Endoglycosidase is an enzyme that releases oligosaccharides from glycoproteins or glycolipids. It may also cleave polysaccharide chains between residues that are not the terminal residue, although releasing oligosaccharides from conjugated protein and lipid molecules is more common. It breaks the glycosidic bonds between two sugar monomer in the polymer. It is different from exoglycosidase that it does not do so at the terminal residue. Hence, it is used to release long carbohydrates from conjugated molecules. If an exoglycosidase were used, every monomer in the polymer would have to be removed, one by one from the chain, taking a long time. An endoglycosidase cleaves, giving a polymeric product.
Abbr
Endo-β-N-acetylglucosaminidase, Recombinant (Clostridium perfringens)
Source
Clostridium perfringens
Form
Supplied in 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.2.1.96
Purification
> 95 % as judged by SDS-PAGE
Unit Definition
One unit is defined as the amount of enzyme required to hydrolyse >95 % of the glycoforms from 10 μg of RNaseB in a total reaction
volume of 10 μL at 37°C in 60 min, where a non-denaturing reaction buffer comprises 50 mM sodium phosphate buffer, pH 6.0, containing 1 mg/mL RNaseB.
Optimum temperature
> 25°C
Synonyms
Endoglycosidase; Endo-β-N-acetylglucosaminidase; EC 3.2.1.96; 231-791-2
