Cat No.
NATE-0220
Description
Endoproteinase Lys-C inactivates the enzyme inositol monophosphatase by cleaving it at a single site directly after Lys 36.
Abbr
Endoproteinase Lys-C, Native (Lysobacter enzymogenes)
Alias
protease I
Species
Lysobacter enzymogenes
Form
lyophilized powder
Enzyme Commission Number
EC 3.4.21.50
CAS No.
72561-05-8
Unit Definition
One unit will hydrolyze 1.0 μmole of N-p-tosyl-Gly-Pro-Lys p-nitroanilide per min at pH 7.7 at 25°C.
Applications
Endoproteinase Lys-C from Lysobacter enzymogenes is useful in the determination of primary structures of proteins. It has been used in a study to investigate the evidence for trisulfide bonds in a recombinant variant of a human IgG2 mon oclonal antibody. Endoproteinase Lys-C has been used for the digestion mon oclonal antibodies for disulfide bond assignment.
Synonyms
EC 3.4.21.50; chromobacter proteinase I; Achromobacter lyticus alkaline proteinase I; protease I; achromopeptidase; lysyl bond specific proteinase; 72561-05-8; Achromobacter proteinase I; Endoproteinase Lys-C
