Cat No.
NATE-1082
Abbr
exo-1,3-β-D-Glucanase, Recombinant (Trichoderma virens)
Species
Trichoderma virens
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.58
Bio-activity
100 U/mg protein (on laminarin) at pH 4.5 and 50°C; 64 U/mg protein (on laminarin) at pH 4.5 and 40°C.
CAS No.
9073-49-8
Molecular Mass
~ 81,700
pH Stability
4.0 - 9.0
Isoelectric point
~ 7.0
Unit Definition
One Unit of exo-1,3-β-glucanase activity is defined as the amount of enzyme required to release one μmole of glucose reducing sugar equivalents per minute from laminarin (Laminaria digitata) (5 mg/mL) in sodium acetate buffer (100 mM) at pH 4.5 and 50°C.
Optimum pH
4.0 - 5.0
Optimum temperature
50°C
Thermal stability
up to 40°C
Stability
> 4 years at 4°C
Preparation Instructions
For assay, this enzyme should be diluted in sodium acetate buffer (20 mM), pH 4.5 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Applications
High purity exo-1,3-beta-Glucanase (Trichoderma virens) (Recombinant) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
EC 3.2.1.58; glucan 1,3-beta-glucosidase; 3-beta-D-glucan glucohydrolase
