Cat No.
NATE-0250
Description
Factor Xa catalyzes the hydrolysis of the Arg-Thr and then Arg-Ile bonds in prothrombin to yield active thrombin. The fairly strict recognition sequence is Ile-Glu (or Asp)-Gly-Arg-↓-X. It may sometimes cleave at other basic residues, depending on the conformation of the target protein. Factor Xa will not cleave if a proline residue follows the arginine of the recognition sequence.
Abbr
Factor Xa, Native (Bovine)
Alias
factor Xa
Source
Bovine plasma
Species
Bovine
Form
aqueous glycerol solution
Enzyme Commission Number
EC 3.4.21.6
CAS No.
9002-05-5
Unit Definition
One unit of activated Factor X will liberate 1.0 μmole of p-nitroanilide from N-benzoyl-L-isoleucyl-L-glutamyl-L-glycyl-L-arginine-p-nitroaniline per minute at pH 8.3 at 37°C.
Optimum pH
7.6-8.0
Optimum temperature
37°C
Applications
Fusion proteins are commonly expressed with a factor Xa cleavable Ile-Glu (or Asp)-Gly-Arg-↓-X sequence. Typically 1 mg of fusion protein can be incubated with 10 μg of factor Xa for 2.5 hours at 37 oc.
Gene Name
F10 coagulation factor X [Bos taurus]
Synonyms
EC 3.4.21.6; 9002-05-5; thrombokinase; prothrombase; prothrombinase; activated blood-coagulation factor X; autoprothrombin C; thromboplastin; plasma thromboplastin; factor Xa; activated Stuart-Prower factor; activated factor X; coagulation factor Xa
GeneID
280787
mRNA Refseq
NM_001080213
Protein Refseq
NP_001073682
UniProt ID
P00743
Chromosome Location
chromosome: 12
Pathway
Common Pathway, organism-specific biosystem; Complement and Coagulation Cascades, organism-specific biosystem; Extrinsic Pathway, organism-specific biosystem
Function
calcium ion binding; phospholipid binding; serine-type endopeptidase activity
