Cat No.
NATE-0837
Description
In enzymology, a feruloyl esterase (EC 3.1.1.73) is an enzyme that catalyzes the chemical reaction: feruloyl-polysaccharide + H2O rightleftharpoons ferulate + polysaccharide. Thus, the two substrates of this enzyme are feruloyl-polysaccharide and H2O, whereas its two products are ferulate and polysaccharide. This enzyme belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds.
Abbr
Feruloyl esterase, Recombinant (Clostridium thermocellum)
Species
Clostridium thermocellum
Form
In 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.1.1.73
Bio-activity
0.5 U/mg protein (on ethyl-ferulate) at pH 6.0 and 50°C. ~ 28 U/mg protein (on FAXX) at pH 6.0 and 60°C.
Molecular Mass
~ 29 kDa
Isoelectric point
~ 6.8
Unit Definition
One Unit of feruloyl esterase activity is defined as the amount of enzyme required to release one μmole of ferulic acid from ethyl-ferulate per minute at pH 6.0 and 50°C.
Optimum pH
4.0 - 7.0
Optimum temperature
50 - 60°C
Stability
> 2 years at 4°C.
Preparation Instructions
For assay, this enzyme should be diluted in sodium phosphate buffer (100 mM), pH 6.0 containing 1.0 mg/ml BSA. Swirl to mix the enzyme immediately prior to use.
Applications
High purity recombinant Feruloyl esterase (Clostridium thermocellum) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
feruloyl esterase; ferulic acid esterase (FAE); hydroxycinnamoyl esterase; hemicellulase accessory enzyme; cinnamoyl ester hydrolase (cinnAE); EC 3.1.1.73; 4-hydroxy-3-methoxycinnamoyl-sugar hydrolase
