Cat No.
NATE-0255
Description
Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab')2 fragments from mouse IgG1.
Abbr
Ficin, Native (Fig tree latex)
Alias
ficin; debricin
Species
Fig tree latex
Form
saline suspension or lyophilized powder
Enzyme Commission Number
EC 3.4.22.3
Bio-activity
> 1.0 units/mg protein
CAS No.
9001-33-6
Molecular Mass
23.8 kDa
Specificity
Extinction Coefficient: E1% = 21.0 (280 nm) pI: 9.0
Unit Definition
One unit will produce a ΔA280 of 1.0 per min at pH 7.0 at 37°C when measuring TCA soluble products from casein in a final volume of 10 mL (1 cm light path).
Synonyms
ficin; debricin; higueroxyl delabarre; EC 3.4.22.3; 9001-33-6; ficain
