Cat No.
NATE-1067
Description
Alpha-Fucosidase is an enzyme that breaks down fucose. Fucosidosis is an autosomal recessive lysosomal storage disease caused by defective alpha-L-fucosidase with accumulation of fucose in the tissues. Different phenotypes include clinical features such as neurologic deterioration, growth retardation, visceromegaly, and seizures in a severe early form; coarse facial features, angiokeratoma corporis diffusum, spasticity and delayed psychomotor development in a longer surviving form; and an unusual spondylometaphyseoepiphyseal dysplasia in yet another form.
Abbr
FUCA, Recombinant (Homo sapiens)
Alias
FUCA
Species
Homo sapiens
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.51
Bio-activity
7 U/mg protein (on pNP-α-L-fucopyranoside) at pH 4.0 and 25°C; ~ 23 U/mg protein (on pNP-α-L-fucopyranoside) at pH 4.0 and 37°C; ~ 68 U/mg protein (on pNP-α-L-fucopyranoside) at pH 4.0 and 50°C.
CAS No.
9037-65-4
Molecular Mass
~ 52,000
pH Stability
4.0-9.0
Isoelectric point
~ 6.3
Specificity
Broad specificity; hydrolysis of terminal non-reducing α-(1-2,3,4,6)-linked L-fucose residues from glycoproteins and oligosaccharides.
Unit Definition
One Unit of α-L-fucosidase activity is defined as the amount of enzyme required to release one μmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-α-L-fucopyranoside (1 mM) in sodium acetate buffer (100 mM) at pH 4.0 at the temperatures indicated.
Optimum pH
3.0-4.0
Optimum temperature
50°C
Thermal stability
up to 60°C
Stability
> 2 years at 4°C.
Preparation Instructions
For assay, this enzyme should be diluted in sodium acetate buffer (100 mM), pH 4.0 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Applications
High purity α-(1-2,3,4,6)-L-Fucosidase (Homo sapiens) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
α-L-fucoside fucohydrolase; Alpha-Fucosidase; FUCA1; FUCA; EC 3.2.1.51
