Cat No.
NATE-1066
Description
Alpha-Fucosidase is an enzyme that breaks down fucose. Fucosidosis is an autosomal recessive lysosomal storage disease caused by defective alpha-L-fucosidase with accumulation of fucose in the tissues. Different phenotypes include clinical features such as neurologic deterioration, growth retardation, visceromegaly, and seizures in a severe early form; coarse facial features, angiokeratoma corporis diffusum, spasticity and delayed psychomotor development in a longer surviving form; and an unusual spondylometaphyseoepiphyseal dysplasia in yet another form.
Abbr
FUCA, Recombinant (T. maritima)
Alias
FUCA
Species
Thermotoga maritima
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.51
Bio-activity
At pH 5.0: 2.6 U/mg protein (25°C); ~4.2 U/mg protein (37°C); ~15.0 U/mg protein (60°C); ~195 U/mg protein (95°C).
Molecular Mass
~ 54,351
pH Stability
4.0-6.0
Isoelectric point
~ 6.1
Unit Definition
One Unit of α-fucosidase activity is defined as the amount of enzyme required to release one μmole of p-nitrophenol (p-NP) per minute from p-nitrophenyl-α-Lfucopyranoside (0.476 mM) in citrate (50 mM)/phosphate (100 mM) buffer, pH 5.0 at the temperatures indicated.
Optimum pH
5
Optimum temperature
95°C
Thermal stability
stable up to 100°C
Preparation Instructions
Swirl to mix the enzyme immediately prior to use.
Applications
High purity recombinant alpha-Fucosidase (Thermostable) (T. maritima) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
α-L-fucoside fucohydrolase; Alpha-Fucosidase; FUCA1; FUCA; EC 3.2.1.51
