Cat No.
NATE-1092
Description
β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.
Abbr
GUSB, Recombinant (E. coli)
Alias
GUSB
Species
E. coli
Form
Supplied in solution (Tris.HCl / NaCl / EDTA).
Enzyme Commission Number
EC 3.2.1.31
Bio-activity
30000 U/mg protein (on phenolphthalein-β-D-glucuronide) at pH 6.8 and 37°C
Molecular Mass
~ 82,600
Isoelectric point
~ 5.4
Specificity
Hydrolysis of non-reducing terminal β-D-glucuronic acid residues from glycoproteins and oligosaccharides of glycoconjugates.
Unit Definition
One Unit of β-D-glucuronosidase activity is defined as the amount of enzyme required to release one μg of phenolphthalein per hour from phenolphthalein-β-D-glucuronide (0.5 mM) in sodium phosphate buffer (100 mM) at pH 6.8 and 37°C.
Optimum pH
5.0 - 7.5
Thermal stability
up to 50°C
Preparation Instructions
For assay, this enzyme should be diluted in sodium phosphate buffer (100 mM), pH 6.8 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Applications
High purity recombinant beta-Glucuronidase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
β-glucuronide glucuronohydrolase glucuronidase; exo-β-D-glucuronidase; ketodase; EC 3.2.1.31; 9001-45-0; β-D-glucuronoside glucuronosohydrolase; GUSB
