Cat No.
NATE-0355
Description
Pyrophosphatase (or inorganic pyrophosphatase) is an enzyme (EC 3.6.1.1) that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. This is a highly exergonic reaction, and therefore can be coupled to unfavorable biochemical transformations in order to drive these transformations to completion. The functionality of this enzyme plays a critical role in lipid metabolism (including lipid synthesis and degradation), calcium absorption and bone formation, and DNA synthesis,as well as other biochemical transformations.
Abbr
Inorganic pyrophosphatase, Recombinant (E. coli)
Source
E. coli
Species
Escherichia coli
Form
Lyophilized powder in Tris-buffered salts containing protease inhibitors
Enzyme Commission Number
EC 3.6.1.1
Bio-activity
> 800 units/mg protein
CAS No.
9024-82-2
Purification
> 90%
Unit Definition
One unit will release 1.0 μmole of inorganic orthophosphate per minute at pH 9 at 25°C.
Applications
Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PPi + H2O → 2Pi. It plays an important role in protein, RNA, and DNA synthesis.
Warnings
A homohexameric protein containing 175 amino acid residues per subunit, its activity is Mg2+ dependent. It is a relatively thermostable protein.
Synonyms
Pyrophosphate phosphohydrolase; inorganic pyrophosphatase; EC 3.6.1.1; 9024-82-2; iphosphate phosphohydrolase
