Cat No.
NATE-0850
Description
Isopullulanase (EC 3.2.1.57) is an enzyme with system name pullulan 4-glucanohydrolase (isopanose-forming). This enzyme catalyses the following chemical reaction: Hydrolysis of pullulan to isopanose (6-alpha-maltosylglucose). The enzyme has no activity on starch.
Abbr
Isopullulanase, Recombinant (Aspergillus niger)
Species
Aspergillus niger
Form
In 3.2 M ammonium sulphate
Enzyme Commission Number
EC 3.2.1.57
Bio-activity
42 U/mg protein (on pullulan) at pH 3.5 and 40°C.
Molecular Mass
~ 60.5 kDa
pH Stability
3.0 - 9.0 (> 75% control activity after 24 hours at 4°C)
Isoelectric point
~ 5.2
Specificity
Hydrolysis of pullulan to isopanose (6-α-maltosylglucose).
Unit Definition
One Unit of isopullulanase activity is defined as the amount of enzyme required to release one μmole of glucose reducing sugar equivalents per minute from pullulan (10 mg/mL) in formic acid buffer (100 mM), pH 3.5 at 40°C.
Optimum pH
3.0 - 3.5
Optimum temperature
40°C
Stability
> 2 years at 4°C.
Preparation Instructions
For assay, this enzyme should be diluted in formic acid buffer (100 mM), pH 3.5 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Applications
High purity recombinant Isopullulanase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Synonyms
Isopullulanase; EC 3.2.1.57; pullulan 4-glucanohydrolase (isopanose-forming)
