Cat No.
NATE-0853
Description
Keratinase is a particular class of extracellular proteolytic inducible enzyme with the capability of degrading insoluble keratin substrates. It is important for hydrolyzing hair, feather, and collagen in sewage system during waste water treatment. It is also useful in food industry, animal feed preparation etc. Insoluble feather keratin from poultry industry may be converted by enzymatic hydrolysis to glues, feedstuffs, fertilizers, films or used for the production of rare amino acids serine, cysteine and proline.
Abbr
Keratinase, Recombinant
Source
E. coli BL21
Form
Lyophilized powder
Enzyme Commission Number
EC 3.4.4.25
Bio-activity
300 - 1000 units/mg
Molecular Mass
~39 kDa
pH Stability
5.5 - 12.5
Isoelectric point
8.73
Unit Definition
One unit of enzyme is able to hydrolyze casein resulting in an absorbance value as the Folin-Ciocalteau reagent equivalent to 1 umole (181μg) of tyrosine per minute at pH 7.5 at 37 °C.
Optimum pH
12.5
Optimum temperature
37°- 70°C
Activators
0.10% SDS, 1.0% CTAB, and EDTA
Inhibitors
Tween 20, DMSO, isopropanol, methanol, and ethanol
Preparation Instructions
The enzyme can be solubilized at 0.5-1.0 mg/ml in either sterile water or phosphate buffer. The best activity is seen with freshly prepared solutions. However, single-use aliquots of Keratinase solutions can be stored at -20° C.
Applications
Keratinase was used for enzymatic treatment of elementary body (EB), GAG molecules, and cells in the study of the role glycosaminoglycans (GAGs) in the invasion of host cells by Chlamydia pneumoniae strains.
Synonyms
Keratinase; KerA; Keratinase from Bacillus licheniformis; Keratinolytic protease; EC 3.4.4.25
