Cat No.
NATE-0431
Description
Lyticase hydrolyzes poly-β (1→3)-glucose such as yeast cell wall glucan.
Abbr
Lyticase, Native (Arthrobacter luteus)
Alias
Lyticase
Species
Arthrobacter luteus
Form
lyophilized powder
Bio-activity
> 200 units/mg solid; > 1,500 units/mg protein; > 2,000 units/mg protein, Protein > 20 % by biuret
CAS No.
37340-57-1
Unit Definition
One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25°C, using a suspension of yeast as substrate in a 3 mL reaction mixture.
Applications
Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation. Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species.
Synonyms
Lyticase; 37340-57-1
