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Cat No.
PHAM-180
Description
Ureases (EC 3.5.1.5), functionally, belong to the superfamily of amidohydrolases and phosphotriesterases. It is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. The reaction occurs as follows: (NH2)2CO + H2O → CO2 + 2NH3.
Abbr
Urease (Jack bean)
Alias
Urease
Species
Jack bean
Form
Freeze dried powder
Enzyme Commission Number
EC 3.5.1.5
Bio-activity
GradeⅡ(-201) 100U/mg-solid or more
CAS No.
9002-13-5
Reaction
urea + H2O = CO2 + 2 NH3
Appearance
White amorphous powder, lyophilized
Molecular Mass
approx. 480 kDa
pH Stability
pH 5.5-8.5 (30°C, 17hr)
Michaelis Constant
1.05×10?2M (Urea)
Structure
8 active sites with SH-groups per mole of the enzyme
Isoelectric point
5.0-5.1
Optimum pH
6
Optimum temperature
60°C
Stabilizers
EDTA, glutathione, succinate, BSA
Thermal stability
below 50°C (pH 8.0, 60min)
Stability
Store at-20°C (A decrease in activity of ca.15% may occur within 6 months)
Inhibitors
Heavy metal ions (Ag?,Hg??,etc.)
Applications
This enzyme is useful for enzymatic determination of urea in clinical analysis.
Synonyms
EC 3.5.1.5; Urease
