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Cat No.
DIA-194
Description
Glycoside hydrolases (also called glycosidases or glycosyl hydrolases) assist in the hydrolysis of glycosidic bonds in complex sugars. They are extremely common enzymes with roles in nature including degradation of biomass such as cellulose and hemicellulose, in anti-bacterial defense strategies (e.g., lysozyme), in pathogenesis mechanisms (e.g., viral neuraminidases) and in normal cellular function (e.g., trimming mannosidases involved in N-linked glycoprotein biosynthesis). Together with glycosyltransferases, glycosidases form the major catalytic machinery for the synthesis and breakage of glycosidic bonds.
Abbr
α-Glucosidase (Microorganism)
Alias
maltase
Species
Microorganism
Form
Freeze dried powder
Enzyme Commission Number
EC 3.2.1.20
Bio-activity
GradeⅡ 20U/mg-solid or more
Appearance
White amorphous powder, lyophilized
Molecular Mass
approx. 65 kDa (Gel-filtration and SDS-PAGE)
pH Stability
pH 5.0-9.0
Michaelis Constant
6.3×10??M (p-Nitrophenyl-α-D-glucopyranoside)
Isoelectric point
5.2
Optimum pH
6.0-7.0
Optimum temperature
60°C
Stabilizers
Bovine serum albumin (BSA)
Thermal stability
below 60°C (pH 7.0, 15min)
Stability
Stable at-20°C for at least one year
Inhibitors
Ag?, Hg??, PCMB, MIA
Applications
This enzyme is useful for structural investigations of carbohydrates and for the enzymatic determination of α-amylase when coupled with hexokinase and G-6-P dehydrogenase in clinical analysis.
Synonyms
Alpha-glucosidase; EC 3.2.1.20; maltase; glucoinvertase; glucosidosucrase; maltase-glucoamylase; alpha-glucopyranosidase; glucosidoinvertase; alpha-D-glucosidase; alpha-glucoside hydrolase; alpha-1,4-glucosidase; alpha-D-glucoside glucohydrolase; glycosidases; glycosyl hydrolases; α-Glucosidase
