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Cat No.
DIA-265
Description
Phosphoglucomutase (EC 5.4.2.2) is an enzyme that transfers a phosphate group on an α-D-glucose monomer from the 1' to the 6' position in the forward direction or the 6' to the 1' position in the reverse direction. More precisely, it facilitates the interconversion of glucose 1-phosphate and glucose 6-phosphate.
Abbr
α-phosphoglucomutase (Microbial)
Species
Microbial
Enzyme Commission Number
EC 5.4.2.2
Molecular Mass
MW ~ 59.2 kDa
pH Stability
6.0-9.0 (> 75% control activity after 24 hours at 4°C)
Composition
Amino acid composition has been reported by Squire et al. (1964).
Isoelectric point
pI ~ 5.7
Thermal stability
up to 40°C (> 90% control activity after 15 min.)
Warnings
The enzyme is supplied as a solution containing 50% glycerol plus 0.02% (w/v) sodium azide and should be stored at 4°C. For assay, this enzyme should be diluted in glycylglycine buffer (100 mM), pH 7.4 containing 0.5 mg/mL BSA. Swirl to mix the enzyme suspension immediately prior to use.
Synonyms
α-phosphoglucomutase; Phosphoglucomutase; EC 5.4.2.2; Phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent); Glucose phosphomutase; Phosphoglucose mutase
