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Cat No.
DIA-271
Description
Adenosine deaminase is an enzyme (EC 3.5.4.4) involved in purine metabolism. It is needed for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues. Present in virtually all mammalian cells, its primary function in Humans is the development and maintenance of the immune system.
Abbr
ADA (Calf Spleen)
Alias
ADA
Source
Calf Spleen
Species
Calf
Formula
Suspension in 3.2 M (NH4)2SO4,0.01 M potassium phosphate, pH 6.0
Enzyme Commission Number
EC 3.5.4.4
Bio-activity
150~200U/mg protein
CAS No.
9026-93-1
Molecular Mass
33 kDa (SDS-PAGE)
pH Stability
4.0~10.0
Michaelis Constant
2.2x10-5M (androsterone)
Isoelectric point
4.85
Unit Definition
One unit will deaminate one micromole of adenosine to inosine per minute at pH 7.4 at 25°C.
Optimum pH
7.4
Optimum temperature
75°C
Thermal stability
< 60°C (pH7.4, 20min)
Inhibitors
Ag+, Hg2+,Cu2+
Preparation Instructions
The enzyme preparation may be dilutedin water or buffer to a protein concentration of 1mg/ml, producing a clear, colorless solution.
Synonyms
ADA; adenosine deaminase; adenosine aminohydrolase; 9026-93-1; EC 3.5.4.4
