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Cat No.
DIA-135
Description
Sterol esterase belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is steryl-ester acylhydrolase. This enzyme participates in bile acid biosynthesis.
Abbr
Cholesterol Esterase (Microorganism)
Species
Microorganism
Enzyme Commission Number
EC 3.1.1.13
Bio-activity
GradeIII 5.0U/mg-solid or more
Appearance
Light brown amorphous powder, lyophilized
pH Stability
pH 5.5-10.0 (25°C, 20hr)
Michaelis Constant
5.7×10??M (Linoleate), 7.2×10??M (Oleate)
Optimum pH
6
Optimum temperature
40°C
Stabilizers
Mg??, bovine serum albumin
Thermal stability
below 40°C (pH 7.0, 15min)
Stability
Store at-20°C
Inhibitors
Hg??, Cu??
Applications
This enzyme is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase in clinical analysis.
Synonyms
cholesterol esterase; cholesteryl ester synthase; triterpenol esterase; cholesteryl esterase; cholesteryl ester hydrolase; sterol ester hydrolase; cholesterol ester hydrolase; cholesterase; acylcholesterol lipase; EC 3.1.1.13; Sterol esterase
