点击图片查看原图
Cat No.
DIA-133
Description
Sterol esterase belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is steryl-ester acylhydrolase. This enzyme participates in bile acid biosynthesis.
Abbr
Cholesterol Esterase (Schizophyllum commune)
Species
Schizophyllum commune
Enzyme Commission Number
EC 3.1.1.13
Bio-activity
GradeIII 2.0 U/mg-solid or more (containing approx. 20% of stabilizers)
Appearance
Light brown amorphous powder, lyophilized
Molecular Mass
approx. 130 kDa
pH Stability
pH 2.5-7.5 (25°C, 20hr)
Michaelis Constant
3.9×10??M (Linoleate), 9.2×10??M (Palmitate), 6.3×10??M (Decylate), 8.8×10??M (Propionate)
Isoelectric point
4.1±0.1
Optimum pH
4.8-8.0 (Cholesterol linoleate), 5.0 (serum)
Optimum temperature
55-60°C
Stabilizers
Na-Cholate
Thermal stability
below 55°C (pH 5.5, 10min)
Stability
Store at-20°C
Inhibitors
Heavy metal ions (Hg??, Ag?, Fe???)
Applications
This enzyme is useful for enzymatic determination of total cholesterol when coupled with cholesterol oxidase in clinical analysis.
Synonyms
cholesterol esterase; cholesteryl ester synthase; triterpenol esterase; cholesteryl esterase; cholesteryl ester hydrolase; sterol ester hydrolase; cholesterol ester hydrolase; cholesterase; acylcholesterol lipase; EC 3.1.1.13; Sterol esterase
