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Cat No.
DIA-406
Description
Recombinant Cholesterol Oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. This enzyme participates in bile acid biosynthesis.
Abbr
CHOD, Recombinant (E. coli)
Alias
CHOD
Source
E. coli
Species
E. coli
Enzyme Commission Number
EC 1.1.3.6
Bio-activity
> 5 U/mg lyophilizate
Appearance
Yellow lyophilizate
Molecular Mass
ca. 58 kDa
pH Stability
5.0–10.0
Michaelis Constant
3.5 x 10^-4 M (cholesterol)
Structure
monomer of 55 kDa (SDS-PAGE)
Unit Definition
One unit (U) is defined as the amount of enzyme which produces 1 μmol of hydrogen peroxide per min at 37°C and pH 7.0.
Optimum pH
7
Optimum temperature
50°C
Thermal stability
below 55°C
Inhibitors
ionic detergents, Ag+, Hg2+
Synonyms
EC 1.1.3.6, cholesterol-O2 oxidoreductase; 3β-hydroxy steroid oxidoreductase; 3β-hydroxysteroid:oxygen oxidoreductase; 9028-76-6
