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Cat No.
DIA-138
Description
Recombinant Cholesterol Oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. This enzyme participates in bile acid biosynthesis.
Abbr
Cholesterol Oxidase (Microorganism)
Species
Microorganism
Enzyme Commission Number
EC 1.1.3.6
Bio-activity
GradeIII 12U/mg-solid or more
Appearance
Yellowish amorphous powder, lyophilized
Molecular Mass
approx. 55 kDa (by gel-filtration)
pH Stability
pH 5.0-10.0 (25°C, 20hr)
Michaelis Constant
3.0×10??M(Cholesterol)
Optimum pH
7.0-8.0
Optimum temperature
60°C
Stabilizers
Bovine serum albumin, amino acids
Thermal stability
below 60°C (pH 7.0, 15min)
Stability
Stable at-20°C for at least 9 months
Inhibitors
Ionic detergents, Hg??
Applications
This enzyme is useful for enzymatic determination of cholesterol in serum when coupled with cholesterol esterase in clinical analysis.
Synonyms
Cholesterol-O2 oxidoreductase; 3 beta-Hydroxy steroid oxidoreductase; 3β-hydroxysteroid: oxygen oxidoreductase; cholesterol: oxygen oxidoreductase; cholesterol oxidase; EC 1.1.3.6
