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Cat No.
DIA-141
Description
Creatine Amidinohydrolase catalyzes the hydrolytic reaction converting creatine to sarcosine and urea. The enzyme is purified from a microorganism. The molecular weight of the enzyme is approximately 67,000. The enzyme is useful for the enzymatic assay of creatine and creatinine when coupled with other related enzymes. creatine + H2O → sarcosine + urea
Abbr
Creatinase, Native (Microorganism)
Species
Microorganism
Enzyme Commission Number
EC 3.5.3.3
Bio-activity
GradeⅡ 4.0U/mg-solid or more
CAS No.
37340-58-2
Appearance
White amorphous powder, lyophilized
Molecular Mass
approx. 67,000 (by gel filtration)
pH Stability
pH 4.0-10.0 (25°C, 20hr)
Michaelis Constant
4.5×10?3 M (Creatine)
Structure
2 subunits per mol of enzyme
Isoelectric point
4.5±0.1
Optimum pH
6.5-7.5
Optimum temperature
40-50°C
Stabilizers
Sugars, EDTA
Thermal stability
below 50°C (pH 7.5, 30min)
Stability
Stable at -20°C for at least one year
Inhibitors
Hg??, Cu??, Ag?, SH reagent (NEM), PCMB
Applications
This enzyme is useful for enzymatic determination of creatine and creatinine when coupled with creatinine amidohydrolase, sarcosine oxidase, and formaldehyde dehydrogenase in clinical analysis.
Synonyms
Creatine amidohydrolase; Creatinase; EC 3.5.3.3
