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Cat No.
DIA-130
Description
Creatinine Amidohydrolase catalyzes the hydrolytic reaction converting creatinine to creatine. The enzyme is purified from a microorganism. The molecular size of the enzyme is approximately 175,000. The enzyme is useful for the enzy-matic assay of creatinine when coupled with other related enzymes. Creatinine + H2O → Creatine.
Abbr
Creatinine amidohydrolase (Pseudomonas sp.)
Species
Pseudomonas sp.
Form
Lyophilized powder containing sucrose and BSA as stabilizers
Enzyme Commission Number
EC 3.5.2.10
Bio-activity
> 250U/mg protein
CAS No.
9025-13-2
Molecular Mass
175 kDa
pH Stability
pH 7.5-9.0 (5°C, 16hr)
Michaelis Constant
3.2 x 10￣2M (Creatinine), 5.7 x 10￣2M (Creatine)
Structure
6 subunits per mol of enzyme (One mol of zinc is bound to each subunit)
Isoelectric point
4.7
Unit Definition
One unit will hydrolyze 1.0 mmole of creatinine to creatine per min at pH 8.0 and 25 °C
Optimum pH
6.5-7.5
Optimum temperature
70°C
Thermal stability
Below 70°C (pH 7.5, 30 min)
Inhibitors
Ag+, Hg++, N-bromosuccinimide, EDTA
Applications
This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis.
Gene Name
GSU1722 creatinine amidohydrolase (Geobacter sulfurreducens PCA)
Synonyms
creatininase; creatinine hydrolase; creatinine amidohydrolase; EC 3.5.2.10; 9025-13-2
GeneID
2685478
Protein Refseq
NP_952772
Function
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides.
