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Cat No.
DIA-192
Description
In enzymology, a quinoprotein glucose dehydrogenase (EC 1.1.5.2) is an enzyme that catalyzes the chemical reaction: D-glucose + ubiquinone ?D-glucono-1,5-lactone + ubiquinol. Thus, the two substrates of this enzyme are D-glucose and ubiquinone, whereas its two products are D-glucono-1,5-lactone and ubiquinol. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with a quinone or similar compound as acceptor. This enzyme participates in pentose phosphate pathway. It employs one cofactor, PQQ.
Abbr
Glucose Dehyrogenase, Native (Microorganism)
Species
Microorganism
Form
Freeze dried powder
Enzyme Commission Number
EC 1.1.5.2
Bio-activity
GradeⅢ 500 U/mg-solid or more
Appearance
Purple amorphous powder, lyophilized
Molecular Mass
approx. 100 kDa (by gel filtration)
pH Stability
pH 3.5-8.5 (25°C, 16hr)
Michaelis Constant
4.8 mM (D-Glucose)
Optimum pH
7
Optimum temperature
37°C
Stabilizers
Ca??, BSA
Thermal stability
below 50°C (pH 7.5, 30min)
Stability
Store at -20°C
Inhibitors
Cu??, Pb??, Ag?
Applications
This enzyme is useful for enzymatic determination of D-Glucose.
Synonyms
Glucose Dehyrogenase; EC 1.1.5.2; D-glucose:ubiquinone oxidoreductase; D-glucose:(pyrroloquinoline-quinone) 1-oxidoreductase; glucose dehydrogenase (PQQ-dependent); glucose dehydrogenase (pyrroloquinoline-quinone); quinoprotein D-glucose dehydrogenase
