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Cat No.
DIA-407
Description
Glucose-6-phosphate dehydrogenase (G6PD or G6PDH) (EC 1.1.1.49) is a cytosolic enzyme that catalyzes the chemical reaction:D-glucose 6-phosphate + NADP+ ? 6-phospho-D-glucono-1,5-lactone + NADPH + H+. This enzyme is in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH).
Abbr
G6PDH, Recombinant (E. coli)
Source
E. coli
Species
E. coli
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 1.1.1.49
Bio-activity
172 U/mg
Concentration
~ 1,250 U/ml
Molecular Mass
~ 56,770
Isoelectric point
~ 5.8
Unit Definition
One Unit of glucose 6-phosphate dehydrogenase (G6PDH) is defined as the amount of enzyme required to produce one μmole of NADPH from NADP+ per minute.
Optimum pH
7.6
Optimum temperature
25°C
Stability
> 2 years
Preparation Instructions
For assay, this enzyme should be diluted in 200 mM Imidazole buffer, pH 7.6 containing 1 mg/mL BSA. Swirl to mix the enzyme suspension immediately prior to use.
Synonyms
EC 1.1.1.49; NADP-glucose-6-phosphate dehydrogenase; Zwischenferment; D-glucose 6-phosphate dehydrogenase; glucose 6-phosphate dehydrogenase (NADP); NADP-dependent glucose 6-phosphate dehydrogenase; 6-phosphoglucose dehydrogenase; Entner-Doudoroff enzyme; glucose-6-phosphate 1-dehydrogenase; G6PDH; GPD; glucose-6-phosphate dehydrogenase; 9001-40-5
