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Cat No.
DIA-287
Description
Recombinant oxidoreductase that catalyzes the interconversion of glycerol 3-phosphate to dihydroxyacetone phosphate. Take advantage of the enhanced liquid stability of this enzyme. Rely on the proven diagnostic quality of this product.
Abbr
Chemically modified GPO, Recombinant (E. coli)
Alias
GPO
Species
E. coli
Bio-activity
>10 U/mg lyophilizate (+37°C, L-α-glycerol phosphate); Specific activity (+25°C): >40 U/mg protein
Appearance
Green yellow amorphous lyophilizate
Molecular Mass
75 kD (SDS-PAGE); 74 kD (gel filtration, Sephadex G 150)
pH Stability
6.5-8.5
Michaelis Constant
K-phosphate buffer, 0.1 mol/l; pH 7.5: 1.36 x 10-2 mol/l (o-dianisidine assay)
Tris buffer, 0.1 mol/l; pH 7.6: 2.90 x 10-3 mol/l (o-dianisidine assay)
Tris buffer, 0.1 mol/l; pH 8.1: 1.40 x 10-3 mol/l (PAP assay)
Isoelectric point
~4.2
Specificity
Glycerol phosphate oxidase reacts highly specific with L-α-glycerol phosphate.
Optimum pH
8.0-8.5
Stability
At +2 to +8°C within specification range for 12 months. Store dry.
Inhibitors
Ag, Hg-salts and SDS
Applications
Use Glycerol-3-phosphate Oxidase in diagnostic tests for the determination of triglycerides together with Glycerol Kinase and Lipoprotein Lipase.
Synonyms
glycerol-3-phosphate oxidase; sn-glycerol-3-phosphate: oxygen 2-oxidoreductase; glycerol phosphate oxidase; glycerol-1-phosphate oxidase; glycerol phosphate oxidase; L-alpha-glycerophosphate oxidase; alpha-glycerophosphate oxidase; L-alpha-glycerol-3-phosphate oxidase; GPO
